Abstract
Plasma amyloid‐beta (Aβ) has long been investigated as a blood biomarker candidate for Cerebral Amyloid Angiopathy (CAA), however previous findings have been inconsistent which could be attributed to the use of less sensitive assays. This study investigates plasma Aβ alterations between pre‐symptomatic Dutch‐type hereditary CAA (D‐CAA) mutation‐carriers (MC) and non-carriers (NC) using two Aβ measurement platforms. Seventeen pre‐symptomatic members of a D‐ CAA pedigree were assembled and followed up 3–4 years later (NC = 8;MC = 9). Plasma Aβ1‐40 and Aβ1‐42 were cross‐sectionally and longitudinally analysed at baseline (T1) and follow‐up (T2) and were found to be lower in MCs compared to NCs, cross‐sectionally after adjusting for covari-ates, at both T1(Aβ1‐40: p = 0.001; Aβ1‐42: p = 0.0004) and T2 (Aβ1‐40: p = 0.001; Aβ1‐42: p = 0.016) employing the Single Molecule Array (Simoa) platform, however no significant differences were observed using the xMAP platform. Further, pairwise longitudinal analyses of plasma Aβ1‐40 revealed decreased levels in MCs using data from the Simoa platform (p = 0.041) and pairwise longitudinal analyses of plasma Aβ1‐42 revealed decreased levels in MCs using data from the xMAP platform (p = 0.041). Findings from the Simoa platform suggest that plasma Aβ may add value to a panel of biomarkers for the diagnosis of pre‐symptomatic CAA, however, further validation studies in larger sample sets are required.
Keywords
[RSTDPub], Amyloid‐beta, Blood biomarkers, Cerebral amyloid angiopathy, Early diagnosis, Hereditary cerebral haemorrhage with amyloidosis—Dutch type, Plasma amyloid‐beta, Single molecule array platform
Document Type
Journal Article
Date of Publication
2021
Volume
22
Issue
6
Publication Title
International Journal of Molecular Sciences
Publisher
MDPI
School
School of Medical and Health Sciences
RAS ID
39617
Funders
National Health and Medical Research Council National Institute of Health Dominantly Inherited Alzheimer Network study
Grant Number
NHMRC Number : APP1129627
Grant Link
http://purl.org/au-research/grants/nhmrc/1129627
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
First Page
1
Last Page
12
Comments
Chatterjee, P., Tegg, M., Pedrini, S., Fagan, A. M., Xiong, C., Singh, A. K., ... & Martins, R. N. (2021). Plasma amyloid‐beta levels in a pre‐symptomatic dutch‐type hereditary cerebral amyloid angiopathy pedigree: A cross‐sectional and longitudinal investigation. International Journal of Molecular Sciences, 22(6), article 2931. https://doi.org/10.3390/ijms22062931