Document Type
Journal Article
Publication Title
Alzheimer's Research & Therapy
Volume
13
Issue
1
PubMed ID
33863377
Publisher
Springer Nature
School
School of Medical and Health Sciences
RAS ID
35847
Funders
National Health and Medical Research Council / Commonwealth Scientific and Industrial Research Organisation
Abstract
Background: CSF biomarkers are well-established for routine clinical use, yet a paucity of comparative assessment exists regarding CSF extraction methods during lumbar puncture. Here, we compare in detail biomarker profiles in CSF extracted using either gravity drip or aspiration. Methods: Biomarkers for β-amyloidopathy (Aβ1–42, Aβ1–40), tauopathy (total tau), or synapse pathology (BACE1, Neurogranin Trunc-p75, α-synuclein) were assessed between gravity or aspiration extraction methods in a sub-population of the Australian Imaging, Biomarkers and Lifestyle (AIBL) study (cognitively normal, N = 36; mild cognitive impairment, N = 8; Alzheimer’s disease, N = 6). Results: High biomarker concordance between extraction methods was seen (concordance correlation > 0.85). Passing Bablock regression defined low beta coefficients indicating high scalability. Conclusions: Levels of these commonly assessed CSF biomarkers are not influenced by extraction method. Results of this study should be incorporated into new consensus guidelines for CSF collection, storage, and analysis of biomarkers.
DOI
10.1186/s13195-021-00812-9
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Comments
Doecke, J. D., Francois, C., Fowler, C. J., Stoops, E., Bourgeat, P., Rainey-Smith, S. R., ... Vanderstichele, H. M. (2021). Core Alzheimer’s disease cerebrospinal fluid biomarker assays are not affected by aspiration or gravity drip extraction methods. Alzheimer's Research & Therapy, 13(1), article 79. https://doi.org/10.1186/s13195-021-00812-9