The impact of blood sampling technique, including the use of peripheral intravenous cannula, on haemolysis rates: A cohort study

Document Type

Journal Article

Publication Title

Journal of Clinical Nursing

Volume

30

Issue

13-14

First Page

1916

Last Page

1926

PubMed ID

33829557

Publisher

Wiley

School

School of Nursing and Midwifery

RAS ID

32945

Funders

Western Australian Nurses Memorial Charitable Trust

Comments

Jacob, E., Jacob, A., Davies, H., Jacob, D., Jenkins, M., Husain, M., & Coventry, L. (2021). The impact of blood sampling technique, including the use of peripheral intravenous cannula, on haemolysis rates: A cohort study. Journal of Clinical Nursing, 30(13-14), 1916-1926. https://doi.org/10.1111/jocn.15744

Abstract

Aims: To explore the relationship between blood sampling techniques and haemolysis. Background: Haemolysis rates of blood samples have been thought to be influenced by the method of collection. There is a lack of research evidence available to clearly show the comparative risk of haemolysis across different blood sampling methods, including venepuncture and use of peripheral intravenous cannulas. Design: A prospective cohort study. Reporting followed the STROBE checklist. Methods: A trained observer was used to record blood sampling techniques over a 10-week period between April and June 2019. These records were then linked to pathology haemolysis results. Multivariable logistic regression was used to model patient and blood draw characteristics affecting haemolysis. Results: Most of the blood samples were not haemolysed (n = 324, 87.1%). Multivariable analysis showed haemolysis was associated with increased tourniquet duration and if the level of tube was less than half full. Univariable analysis showed haemolysis was associated with increased age of the patient, the difficulty of cannulation/ venepuncture and increased number of attempts. No difference was found in the haemolysis rate related to the qualification of the blood collector. Conclusion: There was no significant difference in haemolysis rates associated with sampling blood from a PIVC compared with venepuncture. Research should be undertaken to determine whether education on the factors influencing haemolysis is useful in decreasing haemolysis rates. Relevance to clinical practice: There was no association with increased haemolysis rates when drawing blood via venepuncture compared with a peripheral intravenous cannula. Haemolysis of blood samples was associated with increased tourniquet duration, if level of the tube was less than half-filled, increased age of the patient and difficulty of blood draw. Awareness of the risk of haemolysis associated with specific blood sampling methods may assist clinicians to improve care.

DOI

10.1111/jocn.15744

Access Rights

subscription content

Share

 
COinS