Author Identifier

Angela Juhasz

https://orcid.org/0000-0002-4317-2027

Document Type

Journal Article

Publication Title

Frontiers in Nutrition

Publisher

Frontiers

School

School of Science

RAS ID

36149

Comments

Escobar-Correas, S., Broadbent, J. A., Andraszek, A., Stockwell, S., Howitt, C. A., Juhász, A., & Colgrave, M. L. (2021). Perennial ryegrass contains gluten-like proteins that could contaminate cereal crops. Frontiers in Nutrition, 8, article 708122. https://doi.org/10.3389/fnut.2021.708122

Abstract

Background: To ensure safe consumption of gluten-free products, there is a need to understand all sources of unintentional contamination with gluten in the food chain. In this study, ryegrass (Lolium perenne), a common weed infesting cereal crop, is analysed as a potential source of gluten-like peptide contamination. Materials and Methods: Ten ryegrass cultivars were analysed using shot gun proteomics for the presence of proteins from the prolamin superfamily. A relative quantitative assay was developed to detect ryegrass gluten-like peptides in comparison with those found in 10 common wheat cultivars. Results: A total of 19 protein accessions were found across 10 cultivars of rye grass for the protein families of PF00234-Tryp_alpha_amyl, PF13016-Gliadin, and PF03157-Glutenin_HMW. Protein and peptide homology searches revealed that gliadin-like peptides were similar to avenin and gamma-gliadin peptides. A total of20 peptides, characteristic of prolamin superfamily proteins, were selected for liquid chromatography mass spectrometry (LC-MS) with multiple reaction monitoring (MRM). Only two of the monitored peptides were detected with high abundance in wheat, and all others were detected in ryegrass. Glutenin and alpha-amylase/trypsin inhibitor peptides were reported for the first time in ryegrass and were noted to be conserved across the Poaceae family. Conclusion: A suite of gluten-like peptides were identified using proteomics that showed consistent abundance across ryegrass cultivars but were not detected in wheat cultivars. These peptides will be useful for differentiating wheat gluten contamination from rye grass gluten contamination.

DOI

10.3389/fnut.2021.708122

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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