Association between transcription factor 7-like-2 polymorphisms and type 2 diabetes mellitus in a Ghanaian population

Authors

Christian Obirikorang
Evans Asamoah Adu
Enoch Odame Anto, Edith Cowan UniversityFollow
Emmanuel Acheampong, Edith Cowan UniversityFollow
Lawrence Quaye
Brodrick Yeboah Amoah
Max Efui Annani-Akollor
Aaron Siaw Kwakye
Foster Fokuoh
Michael Appiah
Eric Nana Yaw Nyarko
Freeman Aidoo
Eric Adua
Ebenezer Afrifa-Yamoah, Edith Cowan UniversityFollow
Lois Balmer, Edith Cowan UniversityFollow
Wei Wang, Edith Cowan UniversityFollow

Document Type

Journal Article

Publication Title

Sci

Volume

3

Issue

4

Publisher

MDPI

School

School of Science / School of Medical and Health Sciences

RAS ID

39822

Comments

Obirikorang, C., Adu, E. A., Anto, E. O., Acheampong, E., Quaye, L., Amoah, B. Y., ... & Wang, W. (2021). Association between Transcription Factor 7-like-2 Polymorphisms and Type 2 Diabetes Mellitus in a Ghanaian Population. Sci, 3(4), 40. https://doi.org/10.3390/sci3040040

Abstract

Type 2 diabetes mellitus (T2DM) has been strongly associated with single nucleotide polymorphisms (SNPs) in the TCF7L2 gene. This study investigated the association between rs12255372, rs7903146 in the TCF7L2 gene and T2DM in a Ghanaian population. A case-control study design was used for this study. A total of 106 T2DM patients and 110 control participants were selected. Basic data collected included body mass index, blood pressure and socio-demographics. Fasting blood samples were collected and processed for: serum lipid analysis, plasma glucose estimation and plasma HbA1c estimation. Parts of the whole blood samples were used for DNA extraction using a modified salting-out method. Common and allele-specific primers were designed for genotyping using the Modified Tetra-Primer Amplification assay. Associations were evaluated using logistic regression models. The rs7903146 risk variant was significantly associated with 2.16 vs. 4.06 increased odds for T2DM in patients <60 years vs. ≥60 years. Both rs7903146 and rs12255372 were significantly associated with increased odds of T2DM in women, overweight/obese, T2DM negative family history (T2DM-NFH) and low-HDL-C. In a multivariate model, rs7903146 but not rs12255372 was significantly associated with 2.18, 5.01 and 2.25 increased odds of T2DM, under the codominant, recessive and additive model, respectively (p < 0.05). The association between rs7903146 and rs12255372 with T2DM is more highly associated in a subgroup—women and those with T2DM-NFH, yet who have cardiometabolic risk.

DOI

10.3390/sci3040040

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 License.