Multiple reaction monitoring for the accurate quantification of amino acids: Using hydroxyproline to estimate collagen content

Document Type

Book Chapter

Publication Title

Amino Acid Analysis: Methods and Protocols

Publisher

Humana, New York, NY / Springer

School

School of Science

RAS ID

44709

Funders

Cooperative Research Centre for Sheep Innovation

Comments

Colgrave, M. L., Allingham, P. G., Tyrrell, K., & Jones, A. (2019). Multiple reaction monitoring for the accurate quantification of amino acids: Using hydroxyproline to estimate collagen content. In M. A. Alterman (Ed.), Amino Acid Analysis: Methods and Protocols (pp. 33-45). Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9639-1_4

Abstract

Multiple reaction monitoring (MRM) mass spectrometry may be regarded as the gold standard methodology for quantitative mass spectrometry and has been adopted for the analysis of small molecules especially within the pharmaceutical industry. It can also be applied to the analysis of peptides and proteins and to measurement of the basic building blocks of proteins, amino acids. Here we describe the application of MRM mass spectrometry to the measurement of hydroxyproline after acid hydrolysis of various animal tissues. We show that measurement of hydroxyproline provides an accurate and reliable estimate of the collagen content of such tissues and may be a useful indicator of meat tenderness.

DOI

10.1007/978-1-4939-9639-1_4

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