Multiple reaction monitoring for the accurate quantification of amino acids: Using hydroxyproline to estimate collagen content
Abstract
Multiple reaction monitoring (MRM) mass spectrometry may be regarded as the gold standard methodology for quantitative mass spectrometry and has been adopted for the analysis of small molecules especially within the pharmaceutical industry. It can also be applied to the analysis of peptides and proteins and to measurement of the basic building blocks of proteins, amino acids. Here we describe the application of MRM mass spectrometry to the measurement of hydroxyproline after acid hydrolysis of various animal tissues. We show that measurement of hydroxyproline provides an accurate and reliable estimate of the collagen content of such tissues and may be a useful indicator of meat tenderness.
Document Type
Book Chapter
Date of Publication
2019
Publication Title
Amino Acid Analysis: Methods and Protocols
Publisher
Humana, New York, NY / Springer
School
School of Science
RAS ID
44709
Funders
Cooperative Research Centre for Sheep Innovation
Copyright
subscription content
Comments
Colgrave, M. L., Allingham, P. G., Tyrrell, K., & Jones, A. (2019). Multiple reaction monitoring for the accurate quantification of amino acids: Using hydroxyproline to estimate collagen content. In M. A. Alterman (Ed.), Amino Acid Analysis: Methods and Protocols (pp. 33-45). Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9639-1_4