Development of a simplified method of human semen storage for the testing of sperm DNA fragmentation using the Halosperm G2 test kit

Document Type

Journal Article

Publisher

Elsevier

Faculty

Faculty of Health, Engineering and Science

School

School of Medical Sciences

RAS ID

17769

Comments

McEvoy A., Roberts P., Yap K., Matson P. (2014). Development of a simplified method of human semen storage for the testing of sperm DNA fragmentation using the Halosperm G2 test kit. Fertility and Sterility, 102(4), 981-988. Available here

Abstract

Intervention(s): Comparison of sperm DNA fragmentation levels (DFLs) using fresh, snap-frozen and air-dried semen, with air-dried samples stored at different temperatures and time periods to assess DNA stability.Objective: To develop a simple, convenient, and stable storage method for semen before DNA fragmentation testing.Design: Experimental cross-sectional study.Setting: Fertility clinic.Patient(s): 164 male partners of infertile couples.Main Outcome Measure(s): DFL determined by Halosperm G2 kit.Result(s): Results are expressed as mean ± standard error of the mean. The DFLs from fresh and air-dried semen gave comparable results (1.08% ± 0.65%), and from snap-frozen and fresh samples a statistically significant difference (5.5% ± 1.09%). Air-dried semen stored at room temperature for 7 days had a statistically significantly higher DFL compared with semen stored overnight (46.29% ± 9.12%). Samples stored at 4°C for 7 days or 1 day showed no statistically significant difference (0.83% ± 0.82%). DFLs from samples stored for either 1 or 30 days at 4°C showed a statistically significant difference (19.59% ± 5.72%); those stored at -22°C showed no statistically significant difference (0.68% ± 0.53%).Conclusion(s): Air-drying semen is a simple and stable storage method for up to 1 month at -22°C before DNA fragmentation testing.

DOI

10.1016/j.fertnstert.2014.07.737

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