Development of a simplified method of human semen storage for the testing of sperm DNA fragmentation using the Halosperm G2 test kit
Abstract
Intervention(s): Comparison of sperm DNA fragmentation levels (DFLs) using fresh, snap-frozen and air-dried semen, with air-dried samples stored at different temperatures and time periods to assess DNA stability.Objective: To develop a simple, convenient, and stable storage method for semen before DNA fragmentation testing.Design: Experimental cross-sectional study.Setting: Fertility clinic.Patient(s): 164 male partners of infertile couples.Main Outcome Measure(s): DFL determined by Halosperm G2 kit.Result(s): Results are expressed as mean ± standard error of the mean. The DFLs from fresh and air-dried semen gave comparable results (1.08% ± 0.65%), and from snap-frozen and fresh samples a statistically significant difference (5.5% ± 1.09%). Air-dried semen stored at room temperature for 7 days had a statistically significantly higher DFL compared with semen stored overnight (46.29% ± 9.12%). Samples stored at 4°C for 7 days or 1 day showed no statistically significant difference (0.83% ± 0.82%). DFLs from samples stored for either 1 or 30 days at 4°C showed a statistically significant difference (19.59% ± 5.72%); those stored at -22°C showed no statistically significant difference (0.68% ± 0.53%).Conclusion(s): Air-drying semen is a simple and stable storage method for up to 1 month at -22°C before DNA fragmentation testing.
Keywords
Halosperm, semen storage, sperm DNA damage, air dried sample, Article, comparative study, controlled study, cross-sectional study, DNA fragmentation assay, fresh sample, human, male, male infertility, quality control, room temperature, sample fresh air dried sample, sampling, semen analysis, snap frozen air dried sample, snap frozen sample, sperm preservation
Document Type
Journal Article
Date of Publication
1-1-2014
Faculty
Faculty of Health, Engineering and Science
Publisher
Elsevier
School
School of Medical Sciences
RAS ID
17769
Copyright
subscription content
Comments
McEvoy A., Roberts P., Yap K., Matson P. (2014). Development of a simplified method of human semen storage for the testing of sperm DNA fragmentation using the Halosperm G2 test kit. Fertility and Sterility, 102(4), 981-988. Available here