Rapid measurement of urease activity using a potentiometric RuO2 pH sensor for detection of Helicobacter pylori

Authors/Creators

Wade Lonsdale, Edith Cowan UniversityFollow
Devendra Maurya, Edith Cowan UniversityFollow
Magdalena Wajrak, Edith Cowan UniversityFollow
C. Y. Tay
B. J. Marshall
Kamal Alameh, Edith Cowan UniversityFollow

Abstract

The feasibility of using a thin-film RuO₂ (ruthenium oxide) pH sensor for the detection of Helicobacter pylori is investigated. In particular, we demonstrate the ability of the sensor to measure urease activity between 1.0 and 50 U/mL in less than 30 s. The developed sensor exhibited a super-Nernstian response of 77.74 mV/pH and excellent reversibility. The pH sensor⿿s ability to detect H. pylori in real time is demonstrated, and found to be much faster than the rapid urease test.

RAS ID

22863

Document Type

Journal Article

Date of Publication

2017

School

Electron Science Research Institute / School of Science

Copyright

subscription content

Publisher

Elsevier

Comments

Lonsdale, W., Maurya, D. K., Wajrak, M., Tay, C. Y., Marshall, B. J., & Alameh, K. (2017). Rapid measurement of urease activity using a potentiometric RuO 2 pH sensor for detection of Helicobacter pylori. Sensors and Actuators B: Chemical, 242, 1305-1308. https://doi.org/10.1016/j.snb.2016.06.024

Recommended Citation

Lonsdale, W., Maurya, D., Wajrak, M., Tay, C. Y., Marshall, B. J., & Alameh, K. (2017). Rapid measurement of urease activity using a potentiometric RuO2 pH sensor for detection of Helicobacter pylori. DOI: https://doi.org/10.1016/j.snb.2016.06.024