Rapid measurement of urease activity using a potentiometric RuO2 pH sensor for detection of Helicobacter pylori
Electron Science Research Institute / School of Natural Sciences
The feasibility of using a thin-film RuO2 (ruthenium oxide) pH sensor for the detection of Helicobacter pylori is investigated. In particular, we demonstrate the ability of the sensor to measure urease activity between 1.0 and 50 U/mL in less than 30 s. The developed sensor exhibited a super-Nernstian response of 77.74 mV/pH and excellent reversibility. The pH sensors ability to detect H. pylori in real time is demonstrated, and found to be much faster than the rapid urease test.