Intracytoplasmic sperm injection using hyaluronicacid or polyvinylpyrrolidone: A time-lapse siblingoocyte study

Document Type

Journal Article

Publication Title

Human Fertility

Publisher

Taylor & Francis

Place of Publication

United Kingdom

School

School of Medical and Health Sciences

RAS ID

25054

Comments

Liu, Y., Feenan, K., Chapple, V., Roberts, P., & Matson, P. (2017). Intracytoplasmic sperm injection using hyaluronicacid or polyvinylpyrrolidone: A time-lapse siblingoocyte study. Human Fertility, 22(1), 39-45. https://doi.org/10.1080/14647273.2017.1366077

Abstract

This study evaluated the effect of sperm selection and intracytoplasmic sperm injection (ICSI) on subsequent fertilization and embryo development using the hyaluronic acid-based SpermSlowTM (HA-ICSI) compared to injection with polyvinylpyrrolidone (PVP-ICSI). A total of 206 metaphase II oocytes were collected from 21 prospectively enrolled ICSI cycles at Fertility North between July 2014 and March 2015. Sibling oocytes were randomized into HA-ICSI and PVP-ICSI (n¼103 per group). Subsequent fertilization outcomes and embryo development in terms of qualitative and quantitative time-lapse measures following three-day culture in the EmbryoscopeTM were compared. HA-ICSI resulted in significantly lower abnormal fertilization rates (1.9% vs 9.7%, p¼0.017), and a trend towards increased normal fertilization rates (73.8% vs 62.1%, p¼0.073) with increased injection time (2.5 vs 2.1 min, p¼0.001). No differences between HA-ICSI and PVP-ICSI were observed in (a) the proportion of good conventional morphology embryos (50% vs 53.1%, p¼0.712), (b) time-lapse qualitative measures (p>0.05) and (c) time-lapse quantitative measures (p>0.05). In conclusion, HA-ICSI improves fertilization outcomes although sperm injection takes longer to complete. Subsequent embryo development up to day 3 is not affected.

DOI

10.1080/14647273.2017.1366077

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