Antimicrobial activity and phytochemical screening of Alpinia malaccensis (Ran-kiriya) against food-borne bacteria

Document Type

Journal Article

Publication Title

Journal of Applied Microbiology

ISSN

1365-2672

Volume

125

Issue

5

First Page

1276

Last Page

1285

PubMed ID

29972719

Publisher

Published for the Society for Applied Bacteriology by Blackwell Science

School

School of Medical and Health Sciences

RAS ID

29311

Comments

Somarathna, T., Fernando, W. M. A. D. B., Ranaweera, K. K. D. S., Premakumara, G. A. S., Abeysinghe, T., & Weerakkody, N. S. (2018). Antimicrobial activity and phytochemical screening of Alpinia malaccensis (Ran‐kiriya) against food‐borne bacteria. Journal of applied microbiology. 125(5) 1276-1285. Available here

Abstract

AIMS: Investigation of antimicrobial activity and phytochemicals of Alpinia malaccensis (Ran-kiriya) against foodborne bacteria Staphyloccocus aureus, Listeria monocytogenes, Escherichia coli and Salmonella Typhimurium.

METHODS AND RESULTS: Antibacterial activity was tested on the above four foodborne bacteria using agar disc diffusion and broth dilution assay. Alpinia malaccensis rhizome extract chemical composition was determined using gas chromatography-mass spectrometry (GCMS). Active compound was identified using thin-layer chromatography (TLC) and confirmed by nuclear magnetic resonance spectroscopy (NMR). The A. malaccensis rhizome hexane crude extract showed significantly (P < 0·05) higher diameter of inhibition (DIZ) 40 ± 0·52, 38 ± 0·96 and 36 ± 1·45 mm for S. aureusSA113, MSSASS25D and methicillin-resistant S. aureus compared with other tested bacteria. The minimum inhibition concentration and minimum bactericidal concentration were 0·625 and 5 mg ml

CONCLUSIONS: Bioactive 1'ACA of A. malaccensis showed strong antibacterial activity against S. aureus strains including MRSA strain.

SIGNIFICANCE AND IMPACT OF THE STUDY: This study is the first to identify 1'ACA from A. malaccensis. The crude or purified compound could potentially be developed as antimicrobials.

DOI

10.1111/jam.14039

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