The detection of CMV in saliva can mark a systemic infection with CMV in renal transplant recipients
Document Type
Journal Article
Publication Title
International Journal of Molecular Sciences
ISSN
1422-0067
Volume
20
Issue
20
PubMed ID
31652514
Publisher
MDPI
School
School of Medical and Health Sciences
RAS ID
31258
Funders
National Health and Medical Research Council of Australia (NHMRC)
Curtin University
Grant Number
NHMRC Number : 1068652
Abstract
Human cytomegalovirus (CMV) is often transmitted through saliva. The salivary gland is a site of CMV replication and saliva can be used to diagnose congenital CMV infections. CMV replication is monitored in whole blood or plasma in renal transplant recipients (RTR) and associates with clinical disease. However, these assays may not detect replication in the salivary gland and there is little data linking detection in saliva with systemic infection and clinical sequelae. RTR (n = 82) were recruited > 2 years after transplantation. An in-house quantitative PCR assay was used to detect CMV UL54 in saliva samples. CMV DNA was sought in plasma using a commercial assay. Vascular health was predicted using flow mediated dilatation (FMD) and plasma biomarkers. CMV-reactive antibodies were quantified by ELISA and circulating CMV-specific T-cells by an interferon-γ ELISpot assay. Vδ2− γδ T-cells were detected using multicolor flow cytometry reflecting population expansion after CMV infection. The presence of CMV DNA in saliva and plasma associated with plasma levels of antibodies reactive with CMV gB and with populations of circulating Vδ2− γδ T -cells (p < 0.01). T-cells reactive to CMV immediate early (IE)-1 protein were generally lower in patients with CMV DNA in saliva or plasma, but the level of significance varied (p = 0.02–0.16). Additionally, CMV DNA in saliva or plasma associated weakly with impaired FMD (p = 0.06–0.09). The data suggest that CMV detected in saliva reflects systemic infections in adult RTR.
DOI
10.3390/ijms20205230
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Comments
Waters, S., Lee, S., Lloyd, M., Irish, A., & Price, P. (2019). The detection of CMV in saliva can mark a systemic infection with CMV in renal transplant recipients. International Journal of Molecular Sciences, 20(20).
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