Novel method to screen DNA binding proteins for a given DNA fragment
Abstract
The interactions between transcription factors (TFs) and their DNA binding sites are important part of the transcription regulation network. Here we present a simple, low-cost and effective method to identify TFs for a given DNA fragment. The screening was confirmed by Electrophoretic mobility shift assay, and the protein binding to promoter of hCLP46 was purified by SDS-PAGE, further identified to be histone 2a by peptide fingerprinting.
Document Type
Conference Proceeding
Date of Publication
1-1-2016
Publication Title
Medicine and Biopharmaceutical: Proceedings of the 2015 International Conference
Publisher
World Scientific
School
School of Medical and Health Sciences
RAS ID
25319
Copyright
subscription content
Comments
Wang, Y. X., Fang, H. H., Xiao, Z. X., Wu, L. J., Song, M. S., & Wang, W. (2016). Novel method to screen DNA binding proteins for a given DNA fragment. In Medicine and Biopharmaceutical: Proceedings of the 2015 International Conference (pp. 701-707). Singapore, Singapore: World Scientific. https://doi.org/10.1142/9789814719810_0093