Title

Novel method to screen DNA binding proteins for a given DNA fragment

Document Type

Conference Proceeding

Publication Title

Medicine and Biopharmaceutical: Proceedings of the 2015 International Conference

Publisher

World Scientific

School

School of Medical and Health Sciences

RAS ID

25319

Comments

Originally published as: Wang, Y. X., Fang, H. H., Xiao, Z. X., Wu, L. J., Song, M. S., & Wang, W. (2016). Novel method to screen DNA binding proteins for a given DNA fragment. In Medicine and Biopharmaceutical: Proceedings of the 2015 International Conference (pp. 701-707). Singapore, Singapore: World Scientific. Original publication available here

Abstract

The interactions between transcription factors (TFs) and their DNA binding sites are important part of the transcription regulation network. Here we present a simple, low-cost and effective method to identify TFs for a given DNA fragment. The screening was confirmed by Electrophoretic mobility shift assay, and the protein binding to promoter of hCLP46 was purified by SDS-PAGE, further identified to be histone 2a by peptide fingerprinting.

DOI

10.1142/9789814719810_0093

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