Novel method to screen DNA binding proteins for a given DNA fragment

Document Type

Conference Proceeding

Publication Title

Medicine and Biopharmaceutical: Proceedings of the 2015 International Conference

Publisher

World Scientific

School

School of Medical and Health Sciences

RAS ID

25319

Comments

Wang, Y. X., Fang, H. H., Xiao, Z. X., Wu, L. J., Song, M. S., & Wang, W. (2016). Novel method to screen DNA binding proteins for a given DNA fragment. In Medicine and Biopharmaceutical: Proceedings of the 2015 International Conference (pp. 701-707). Singapore, Singapore: World Scientific. https://doi.org/10.1142/9789814719810_0093

Abstract

The interactions between transcription factors (TFs) and their DNA binding sites are important part of the transcription regulation network. Here we present a simple, low-cost and effective method to identify TFs for a given DNA fragment. The screening was confirmed by Electrophoretic mobility shift assay, and the protein binding to promoter of hCLP46 was purified by SDS-PAGE, further identified to be histone 2a by peptide fingerprinting.

DOI

10.1142/9789814719810_0093

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