Novel method to screen DNA binding proteins for a given DNA fragment
Medicine and Biopharmaceutical: Proceedings of the 2015 International Conference
School of Medical and Health Sciences
The interactions between transcription factors (TFs) and their DNA binding sites are important part of the transcription regulation network. Here we present a simple, low-cost and effective method to identify TFs for a given DNA fragment. The screening was confirmed by Electrophoretic mobility shift assay, and the protein binding to promoter of hCLP46 was purified by SDS-PAGE, further identified to be histone 2a by peptide fingerprinting.