Ultrasensitive detection of plasma amyloid-β as a biomarker for cognitively normal elderly individuals at risk of Alzheimer’s disease
Authors
Pratishtha Chatterjee, Edith Cowan UniversityFollow
Mitra Elmi
Kathryn Goozee, Edith Cowan University
Tejal Shah, Edith Cowan UniversityFollow
Hamid R. Sohrabi, Edith Cowan UniversityFollow
Cintia B. Dias
Steve Pedrini, Edith Cowan UniversityFollow
Kaikai Shen
Prita R. Asih
Preeti Dave
Kevin Taddei, Edith Cowan UniversityFollow
Hugo Vanderstichele
Henrik Zetterberg
Kaj Blennow
Ralph N. Martins, Edith Cowan UniversityFollow
Document Type
Journal Article
Publication Title
Journal of Alzheimer's Disease
ISSN
1875-8908
Volume
71
Issue
3
First Page
775
Last Page
783
PubMed ID
31424403
Publisher
IOS Press
School
School of Medical and Health Sciences
RAS ID
31357
Abstract
BACKGROUND: Aberrant amyloid-β (Aβ) deposition in the brain occurs two decades prior to the manifestation of Alzheimer's disease (AD) clinical symptoms and therefore brain Aβ load measured using PET serves as a gold standard biomarker for the early diagnosis of AD. However, the uneconomical nature of PET makes blood markers, that reflect brain Aβ deposition, attractive candidates for investigation as surrogate markers.
OBJECTIVE: Investigation of plasma Aβ as a surrogate marker for brain Aβ deposition in cognitively normal elderly individuals.
METHODS: Plasma Aβ40 and Aβ42 concentrations were measured using the ultrasensitive Single Molecule Array (Simoa) assay in 95 cognitively normal elderly individuals, who have all undergone PET to assess brain Aβ deposition. Based on the standard uptake value ratios (SUVR) obtained from PET imaging, using the tracer 18F-Florbetaben, plasma Aβ was compared between 32 participants assessed to have low brain Aβ load (Aβ-, SUVR
RESULTS: Plasma Aβ42/Aβ40 ratios were lower in the Aβ+ group compared to the Aβ-group. Plasma Aβ40 and Aβ42 levels were not significantly different between Aβ-and Aβ+ groups, although a trend of higher plasma Aβ40 was observed in the Aβ+ group. Additionally, plasma Aβ42/Aβ40 ratios along with the known AD risk factors, age and APOEɛ4 status, resulted in Aβ+ participants being distinguished from Aβ-participants based on an area under the receiver operating characteristic curve shown to be 78%.
CONCLUSION: Plasma Aβ ratios in this study are a potential biomarker for brain Aβ deposition and therefore, for preclinical AD. However, this method to measure plasma Aβ needs further development to increase the accuracy of this promising AD blood biomarker.
DOI
10.3233/JAD-190533
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Comments
Chatterjee, P., Elmi, M., Goozee, K., Shah, T., Sohrabi, H. R., Dias, C. B., ... & Martins, R. (2019). Ultrasensitive detection of plasma amyloid-β as a biomarker for cognitively normal elderly individuals at risk of Alzheimer’s disease. Journal of Alzheimer's Disease, 71(3), 775-783.
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