Simultaneous quantitative analysis of polyphenolic compounds in human plasma by liquid chromatography tandem mass spectrometry
Document Type
Journal Article
Publication Title
Journal of Separation Science
ISSN
1615-9314
Volume
42
Issue
18
First Page
2909
Last Page
2921
PubMed ID
31293055
Publisher
Wiley
School
School of Medical and Health Sciences / School of Science / Centre for Integrative Metabolomics and Computational Biology
RAS ID
30705
Abstract
A diet rich in polyphenolic compounds has recognized health benefits, and as such is routinely monitored as part of dietary intervention studies. A method for the simultaneous determination of 36 phenolic compounds, including phenolic acids and flavonoids, using liquid chromatography and tandem mass spectrometry is described here. The target analytes were quantified based on their specific mass spectral fragments using a selected reaction monitoring approach. A C18 column with embedded aromatic functionality ensured separation of all phenolic compounds studied which included several pairs of isomers. Sample preparation involved the use of β-glucuronidase to release the phenolic compounds from their conjugated forms. The intra-day and inter-day precision and accuracy was less than 7% for all phenolic compounds studied. Recoveries, where plasma was spiked with three different concentrations of the analytes, ranged from 95-115%. The limits of detection and quantification were 0.23-3.89 and 1.15-7.79 nM, respectively. The method was successfully applied to real samples and the range reported for each phenolic compound, with the exception of hydroferulic acid, nordihydroguaiaretic acid, methylgallate, and m-coumaric acid, was at least an order of magnitude higher than the limit of quantification for the method.
DOI
10.1002/jssc.201900339
Access Rights
subscription content
Comments
Shafaei, A., Croft, K., Hodgson, J., & Boyce, M. C. (2019). Simultaneous quantitative analysis of polyphenolic compounds in human plasma by liquid chromatography tandem mass spectrometry. Journal of Separation Science, 42(18), 2909-2921.
Available here.