Immunoglobulin G N-glycan analysis by ultra-performance liquid chromatography

Authors/Creators

Di Liu
Xizhu Xu
Yuejin Li
Jie Zhang
Xiaoyu Zhang
Qihuan Li
Haifeng Hou
Dong Li
Wei Wang, Edith Cowan UniversityFollow
Youxin Wang

Abstract

Glycomics is a new subspecialty in omics system research that offers significant potential in discovering next-generation biomarkers for disease susceptibility, drug target discovery, and precision medicine. Alternative IgG N-glycans have been reported in several common chronic diseases and suggested to have great potential in clinical applications (i.e., biomarkers for diagnosis and prediction of diseases). IgG N-glycans are widely characterized using the method of hydrophilic interaction chromatography (HILIC) ultra-performance liquid chromatography (UPLC). UPLC is a stable detection technology with good reproducibility and high relative quantitative accuracy. In addition, the structure of IgG N-glycan is clearly separated, and glycan composition and relative abundance in plasma are characterized.

RAS ID

31463

Document Type

Journal Article

Date of Publication

1-1-2020

School

School of Medical and Health Sciences

Copyright

subscription content

Publisher

JoVE

Comments

Liu, D., Xu, X., Li, Y., Zhang, J., Zhang, X., Li, Q., ... & Wang, Y. (2020). Immunoglobulin G N-glycan analysis by ultra-performance liquid chromatography. Journal of Visualized Experiments: JoVE, 155, Article e60104. https://doi.org/10.3791/60104

Recommended Citation

Liu, D., Xu, X., Li, Y., Zhang, J., Zhang, X., Li, Q., Hou, H., Li, D., Wang, W., & Wang, Y. (2020). Immunoglobulin G N-glycan analysis by ultra-performance liquid chromatography. DOI: https://doi.org/10.3791/60104