Immunoglobulin G N-glycan analysis by ultra-performance liquid chromatography
Authors/Creators
Di Liu
Xizhu Xu
Yuejin Li
Jie Zhang
Xiaoyu Zhang
Qihuan Li
Haifeng Hou
Dong Li
Wei Wang, Edith Cowan UniversityFollow
Youxin Wang
Abstract
Glycomics is a new subspecialty in omics system research that offers significant potential in discovering next-generation biomarkers for disease susceptibility, drug target discovery, and precision medicine. Alternative IgG N-glycans have been reported in several common chronic diseases and suggested to have great potential in clinical applications (i.e., biomarkers for diagnosis and prediction of diseases). IgG N-glycans are widely characterized using the method of hydrophilic interaction chromatography (HILIC) ultra-performance liquid chromatography (UPLC). UPLC is a stable detection technology with good reproducibility and high relative quantitative accuracy. In addition, the structure of IgG N-glycan is clearly separated, and glycan composition and relative abundance in plasma are characterized.
Document Type
Journal Article
Date of Publication
1-1-2020
Publication Title
Journal of Visualized Experiments : JoVE
Publisher
JoVE
School
School of Medical and Health Sciences
RAS ID
31463
Copyright
subscription content
Comments
Liu, D., Xu, X., Li, Y., Zhang, J., Zhang, X., Li, Q., ... & Wang, Y. (2020). Immunoglobulin G N-glycan analysis by ultra-performance liquid chromatography. Journal of Visualized Experiments: JoVE, 155, Article e60104. https://doi.org/10.3791/60104