Authors/Creators
Utpal Bose
James A. Broadbent
Angéla Juhász, Edith Cowan UniversityFollow
Shaymaviswanathan Karnaneedi
Elecia B. Johnston
Sally Stockwell
Keren Byrne
Vachiranee Limviphuvadh
Sebastian Maurer-Stroh
Andreas L. Lopata
Michelle L. Colgrave, Edith Cowan UniversityFollow
Abstract
© 2021 Elsevier Ltd Insects have been consumed by people for millennia and have recently been proposed as a complementary, sustainable source of protein to feed the world's growing population. Insects and crustaceans both belong to the arthropod family. Crustacean (shellfish) allergies are common and potentially severe; hence, the cross-reactivity of the immune system with insect proteins is a potential health concern. Herein, LC-MS/MS was used to explore the proteome of whole, roasted whole and roasted powdered cricket products. Eight protein extraction protocols were compared using the total number of protein and distinct peptide identifications. Within these data, 20 putative allergens were identified, of which three were arginine kinase (AK) proteoforms. Subsequently, a multiple reaction monitoring MS assay was developed for the AK proteoforms and applied to a subset of extracts. This targeted assay demonstrated that allergen abundance/detectability varies according to the extraction method as well as the food processing method.
RAS ID
32821
Document Type
Journal Article
Date of Publication
2021
Volume
348
Funding Information
1st Singapore-Australia Bilateral Program on Innovations in Food for Precision Health, 2019 Commonwealth Scientific and Industrial Research Organisation James Cook University Agency for Science, Technology and Research
School
School of Science
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Publisher
Elsevier
Recommended Citation
Bose, U., Broadbent, J. A., Juhász, A., Karnaneedi, S., Johnston, E. B., Stockwell, S., Byrne, K., Limviphuvadh, V., Maurer-Stroh, S., Lopata, A. L., & Colgrave, M. L. (2021). Protein extraction protocols for optimal proteome measurement and arginine kinase quantitation from cricket Acheta domesticus for food safety assessment. DOI: https://doi.org/10.1016/j.foodchem.2021.129110
Comments
Bose, U., Broadbent, J. A., Juhász, A., Karnaneedi, S., Johnston, E. B., Stockwell, S., ... Colgrave, M. L. (2021). Protein extraction protocols for optimal proteome measurement and arginine kinase quantitation from cricket Acheta domesticus for food safety assessment. Food Chemistry, 348, article 129110. https://doi.org/10.1016/j.foodchem.2021.129110