Isolation and quantification of plasma circulating tumor DNA from melanoma patients
Abstract
In recent years, circulating tumor DNA (ctDNA) has emerged as a promising prognostic and monitoring biomarker of various cancers, including melanoma. However, sensitive methods are required for its preservation, isolation, and detection. Here we describe a sensitive method for plasma ctDNA isolation using a column-based extraction kit, followed by quantification using a single mutational target with a droplet digital PCR system. This sensitive protocol has been successfully used to quantify diverse mutations present in plasma-derived ctDNA from cancer patients. The full procedure, from blood processing to the analysis of results, takes approximately a day of work.
Document Type
Book Chapter
Date of Publication
2021
Publication Title
Melanoma: Methods and Protocols
Publisher
Springer
School
School of Medical and Health Sciences
RAS ID
36305
Copyright
subscription content
First Page
247
Last Page
263
Comments
Marsavela, G., Reid, A., Gray, E. S., & Calapre, L. (2021). Isolation and quantification of plasma circulating tumor DNA from melanoma patients. In K. M. Hargadon (Ed.), Melanoma: Methods and Protocols (pp. 247-263). Springer.
https://doi.org/10.1007/978-1-0716-1205-7_19