Isolation and quantification of plasma circulating tumor DNA from melanoma patients

Abstract

In recent years, circulating tumor DNA (ctDNA) has emerged as a promising prognostic and monitoring biomarker of various cancers, including melanoma. However, sensitive methods are required for its preservation, isolation, and detection. Here we describe a sensitive method for plasma ctDNA isolation using a column-based extraction kit, followed by quantification using a single mutational target with a droplet digital PCR system. This sensitive protocol has been successfully used to quantify diverse mutations present in plasma-derived ctDNA from cancer patients. The full procedure, from blood processing to the analysis of results, takes approximately a day of work.

RAS ID

36305

Document Type

Book Chapter

Date of Publication

2021

School

School of Medical and Health Sciences

Copyright

subscription content

Publisher

Springer

Comments

Marsavela, G., Reid, A., Gray, E. S., & Calapre, L. (2021). Isolation and quantification of plasma circulating tumor DNA from melanoma patients. In K. M. Hargadon (Ed.), Melanoma: Methods and Protocols (pp. 247-263). Springer.

https://doi.org/10.1007/978-1-0716-1205-7_19

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Link to publisher version (DOI)

10.1007/978-1-0716-1205-7_19