Isolation and quantification of plasma circulating tumor DNA from melanoma patients

Document Type

Book Chapter

Publication Title

Melanoma: Methods and Protocols

First Page

247

Last Page

263

Publisher

Springer

School

School of Medical and Health Sciences

RAS ID

36305

Comments

Marsavela, G., Reid, A., Gray, E. S., & Calapre, L. (2021). Isolation and quantification of plasma circulating tumor DNA from melanoma patients. In K. M. Hargadon (Ed.), Melanoma: Methods and Protocols (pp. 247-263). Springer.

https://doi.org/10.1007/978-1-0716-1205-7_19

Abstract

In recent years, circulating tumor DNA (ctDNA) has emerged as a promising prognostic and monitoring biomarker of various cancers, including melanoma. However, sensitive methods are required for its preservation, isolation, and detection. Here we describe a sensitive method for plasma ctDNA isolation using a column-based extraction kit, followed by quantification using a single mutational target with a droplet digital PCR system. This sensitive protocol has been successfully used to quantify diverse mutations present in plasma-derived ctDNA from cancer patients. The full procedure, from blood processing to the analysis of results, takes approximately a day of work.

DOI

10.1007/978-1-0716-1205-7_19

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