Abstract
© 2020 Elsevier Inc. Modern mass spectrometers can accurately measure thousands of compounds in complex mixtures over a given liquid chromatograph method, depending on desired outcome and method duration. This stream of analytical chemistry has wide ranging application across food, pharma, environmental, forensics, clinical and research. With consistent pressure on both the ruminant production and product industries to face new and substantial challenges, liquid chromatography-mass spectrometry (LC-MS) is an ideal tool to identify, detect and quantify markers of breeding, production and adaption to support both research and industry to overcome these challenges. Herein, we provide a description of the theoretical basis and framework for LC-MS as a rapidly developing technique and highlight its application in measuring cattle and cattle product traits through protein quantitation with specific focus on beta-casein proteoforms.
RAS ID
32934
Document Type
Journal Article
Date of Publication
2-28-2021
Volume
186
Funding Information
University of South Australia
PubMed ID
32956783
School
School of Science
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Publisher
Elsevier
Recommended Citation
Broadbent, J. A., Condina, M. R., & Colgrave, M. L. (2021). Quantitative mass spectrometry-based analysis of proteins related to cattle and their products – focus on cows’ milk beta-casein proteoforms. DOI: https://doi.org/10.1016/j.ymeth.2020.09.011
Comments
This is an author's accepted manuscript of: Broadbent, J. A., Condina, M. R., & Colgrave, M. L. (2021). Quantitative mass spectrometry-based analysis of proteins related to cattle and their products–focus on cows’ milk beta-casein proteoforms. Methods, 186, 112-118. https://doi.org/10.1016/j.ymeth.2020.09.011