Abstract

Background:

The relationship of immunoglobulin G (IgG) glycosylation with diabetes and diabetic nephropathy has been reported, but its role in diabetic retinopathy (DR) remains unclear. We aimed to investigate and validate the association of IgG glycosylation with DR.

Methods:

We analyzed the IgG N-linked glycosylation profile and primarily selected candidate glycans by lasso (least absolute shrinkage and selection operator) regression analysis in the discovery population. The findings were validated in the replication population using a binary logistics model. The association between the significant glycosylation panel and clinical features was illustrated with Spearman's coefficient. The results were confirmed by sensitivity analyses. Results: Among 16 selected glycan candidates using lasso, two IgG glycans (GP15, GP20) and two derived traits (IGP32, IGP54) were identified and validated to be significantly associated with DR (P < .05), and the combined adjusted odds ratios (ORs) were 0.587, 0.613, 1.970, and 0.593, respectively. The glycosylation panel showed a weak correlation with clinical features, except for age. In addition, the results remained consistent when the subjects with prediabetes were excluded from the controls, and the adjusted ORs were 0.677, 0.738, 1.597, and 0.678 in the whole population. Furthermore, in the 1:3 rematched population, a significant association was observed, apart from GP20.

Conclusions:

The IgG glycosylation profile, reflecting an aging and pro-inflammatory status, was significantly associated with DR. The variation in the IgG glycome deserves more attention in diabetic complications.

RAS ID

36599

Document Type

Journal Article

Date of Publication

8-31-2021

Funding Information

Natural Science Fund of China

National Key R&D Program of China

PubMed ID

33491329

School

School of Medical and Health Sciences / Office of PVC (China)

Publisher

Wiley

Comments

This is an author's accepted manuscript of:

Wu, Z., Pan, H., Liu, D., Zhou, D., Li, H., Tao, L., ... Guo, X. (2021). Variation of IgG N-linked glycosylation profile in diabetic retinopathy. Journal of Diabetes, 13(8), 672-680. https://doi.org/10.1111/1753-0407.13160

Download

Share

 
COinS
 

Link to publisher version (DOI)

10.1111/1753-0407.13160