Multi-marker immunomagnetic enrichment of circulating melanoma cells

Authors/Creators

Aaron B. Beasley, Edith Cowan UniversityFollow
Emmanuel Acheampong, Edith Cowan UniversityFollow
Weitao Lin, Edith Cowan UniversityFollow
Elin S. Gray, Edith Cowan UniversityFollow

Abstract

Within the last decade, circulating tumor cells (CTCs) have emerged as a promising biomarker for prognostication, treatment monitoring, and detection of markers of treatment resistance, and their isolation can be used as a minimally invasive means of profiling tumors across multiple body sites. However, CTCs represent a minuscule fraction of the total circulating cells in a patient. Therefore, sensitive isolation methods are needed for the detection and downstream analysis of these cells. Herein we describe a sensitive method for melanoma CTC isolation using a multi-marker immunomagnetic bead method. This method has been purposely optimized to detect CTCs in melanoma patients.

Document Type

Book Chapter

Date of Publication

2021

Volume

2265

PubMed ID

33704717

Publication Title

Melanoma: Methods and Protocols

Publisher

Springer

School

School of Medical and Health Sciences / Graduate Research

RAS ID

36307

Funders

Edith Cowan University Cancer Council of Western Australia

Comments

Beasley, A. B., Acheampong, E., Lin, W., & Gray, E. S. (2021). Multi-marker immunomagnetic enrichment of circulating melanoma cells. In K. M. Hargadon (Ed.), Melanoma: Methods and Protocols (pp. 213-222). Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1205-7_16

Copyright

subscription content

First Page

213

Last Page

222