Multi-marker immunomagnetic enrichment of circulating melanoma cells
Abstract
Within the last decade, circulating tumor cells (CTCs) have emerged as a promising biomarker for prognostication, treatment monitoring, and detection of markers of treatment resistance, and their isolation can be used as a minimally invasive means of profiling tumors across multiple body sites. However, CTCs represent a minuscule fraction of the total circulating cells in a patient. Therefore, sensitive isolation methods are needed for the detection and downstream analysis of these cells. Herein we describe a sensitive method for melanoma CTC isolation using a multi-marker immunomagnetic bead method. This method has been purposely optimized to detect CTCs in melanoma patients.
RAS ID
36307
Document Type
Book Chapter
Date of Publication
2021
Volume
2265
Funding Information
Edith Cowan University Cancer Council of Western Australia
PubMed ID
33704717
School
School of Medical and Health Sciences / Graduate Research
Copyright
subscription content
Publisher
Springer
Comments
Beasley, A. B., Acheampong, E., Lin, W., & Gray, E. S. (2021). Multi-marker immunomagnetic enrichment of circulating melanoma cells. In K. M. Hargadon (Ed.), Melanoma: Methods and Protocols (pp. 213-222). Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1205-7_16