Multi-marker immunomagnetic enrichment of circulating melanoma cells

Document Type

Book Chapter

Publication Title

Melanoma: Methods and Protocols

Volume

2265

First Page

213

Last Page

222

PubMed ID

33704717

Publisher

Springer

School

School of Medical and Health Sciences / Graduate Research

RAS ID

36307

Funders

Edith Cowan University Cancer Council of Western Australia

Comments

Beasley, A. B., Acheampong, E., Lin, W., & Gray, E. S. (2021). Multi-marker immunomagnetic enrichment of circulating melanoma cells. In K. M. Hargadon (Ed.), Melanoma: Methods and Protocols (pp. 213-222). Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1205-7_16

Abstract

Within the last decade, circulating tumor cells (CTCs) have emerged as a promising biomarker for prognostication, treatment monitoring, and detection of markers of treatment resistance, and their isolation can be used as a minimally invasive means of profiling tumors across multiple body sites. However, CTCs represent a minuscule fraction of the total circulating cells in a patient. Therefore, sensitive isolation methods are needed for the detection and downstream analysis of these cells. Herein we describe a sensitive method for melanoma CTC isolation using a multi-marker immunomagnetic bead method. This method has been purposely optimized to detect CTCs in melanoma patients.

DOI

10.1007/978-1-0716-1205-7_16

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