Transcript-based detection of circulating melanoma cells

Document Type

Book Chapter

Publication Title

Melanoma: Methods and Protocols

Volume

2265

First Page

235

Last Page

245

PubMed ID

33704719

Publisher

Springer

School

School of Medical and Health Sciences

RAS ID

36306

Funders

Cancer Research Trust. E.S.G. Cancer Council Western Australia

Comments

Morici, M., Lin, W., & Gray, E. S. (2021). Transcript-based detection of circulating melanoma cells. Melanoma: Methods and Protocols (pp. 235-245). Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1205-7_18

Abstract

Circulating tumor cells (CTCs) are cancer cells shed by the primary tumor or its metastases that circulate in the peripheral blood. CTCs are potential seeds for metastases, and their detection may allow early uncovering of metastatic dissemination and disease prognostication. To fully ascertain the biomarker potential of melanoma CTCs, sensitive and reliable methods are required. Melanoma-specific transcript analysis has been widely utilized as a standard approach for measuring the presence of CTCs. Here we describe a method for the analysis of CTCs through the detection of specific transcripts in CTC-enriched fractions.

DOI

10.1007/978-1-0716-1205-7_18

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Link to publisher version (DOI)

10.1007/978-1-0716-1205-7_18