Date of Award
Bachelor of Science Honours
Faculty of Science, Technology and Engineering
Dr Mary Boyce
Dr Ian Bennett
Several buffers were examined for their ability to separate a complex mixture of phenolic compounds using micellar electrokinetic capillary chromatography (MEKC). The phenolic mixture included simple phenols, phenolic acids and coumarins. Of the different buffers examined, 6 mM borate, 10 mM phosphate, 100 mM SDS, pH 8.3 gave the best separation. The ability of organic modifiers, complexation agents such as α cyclodextrin, β cyclodextrin and mixed SDS I Brij 35 micelles to improve the separation and peak retention time reproducibility of the buffer system was investigated. Baseline separation of the 18 phenolics was achieved by the 6 mM borate, 10 mM phosphate, I 00 mM SDS, 15 mM Brij 35, 10% acetonitrile, pH 8.3 buffer. However this buffer system had poor peak retention time reproducibility. The MEKC system was used to analyse the phenolic content of a range of eucalypt species. Through spiking, several phenolic and related compounds were identified. These compounds included gallic acid, ferulic acid, p-coumaric acid and shikimic acid. GC-MS and HPLC supported the results obtained by CE.
Tromans, A. J. (1996). Development and optimisation of a micellar electrokinetic capillary chromatography method for the separation and identification of phenolics from eucalypt species. https://ro.ecu.edu.au/theses_hons/310