Publication Date

2019

Document Type

Dataset

Publisher

Dryad

School or Research Centre

Centre for Ecosystem Management

Description

Premise of the study: Recent habitat fragmentation is posing a risk to the wavy-leaved smokebush, Conospermum undulatum, a rare plant species endemic to the south-western Western Australia. Microsatellites markers are required to characterize the genetic diversity and structure of the species for conservation purposes and to facilitate ecological studies. Methods and Results: Illumina MiSeq high-throughput sequencing was used to develop 20 novel microsatellite markers for C. undulatum. Polymorphism at each locus was assessed using 72 individuals from three natural populations. Nineteen markers were polymorphic, with the number of alleles per locus ranging from two to 21, and observed and expected heterozygosity ranging from 0.000 to 1.000 and 0.117 to 0.919, respectively. All markers successfully amplified in three congeneric species. Conclusions: The microsatellite markers will be useful for revealing patterns of genetic diversity, dispersal dynamics and hybridization events for C. undulatum to inform future conservation efforts.

Additional Information

This dataset was originally published at:

https://doi.org/10.5061/dryad.f81k3q7

DOI

10.5061/dryad.f81k3q7

Language

Eng

File Format(s)

.xls

File Size

3,005 KB

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Conospermum output

Data outputs from the pipeline generated from NextGeneration sequencing

SMA-Cu1_S55_L001_R1_001

Illumina fastq file output of forward reads for Conospermum undulatum.

SMA-Cu1_S55_L001_R2_001

Illumina fastq file output of reverse reads for Conospermum undulatum.

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Public Domain Dedication 1.0 License.

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