S-methyl cysteine sulfoxide does not ameliorate weight gain or hyperlipidemia in mice fed a high-fat diet

Authors/Creators

Caroline R. Hill, Edith Cowan UniversityFollow
Armaghan Shafaei, Edith Cowan UniversityFollow
Vance B. Matthews
Natalie C. Ward
Kevin D. Croft
Joshua R. Lewis, Edith Cowan UniversityFollow
Jonathan M. Hodgson, Edith Cowan UniversityFollow
Lois Balmer, Edith Cowan UniversityFollow
Lauren C. Blekkenhorst, Edith Cowan UniversityFollow

Abstract

Scope: Higher intake of cruciferous and allium vegetables is associated with lower cardiometabolic risk. Little research has investigated the cardiometabolic effects of S-methyl cysteine sulfoxide (SMCSO), found abundant in these vegetables. This study hypothesizes that SMCSO will blunt development of metabolic syndrome features in mice fed high-fat feed. Methods and results: Fifty C57BL/6 male mice are randomly assigned to standard-chow, high-fat, or high-fat supplemented with low-SMCSO (43 mg kg−1 body weight [BW] day−1), medium-SMCSO (153 mg kg−1 BW day−1), or high-SMCSO (256 mg kg−1 BW day−1) for 12-weeks. High-fat with SMCSO did not prevent diet-induced obesity, glucose intolerance, or hypercholesterolemia. Mice fed high-fat with SMCSO has higher hepatic lipids than mice fed standard-chow or high-fat alone. Urinary SMCSO increases at 6- and 12-weeks in the low-SMCSO group, before reducing 46% and 28% in the medium- and high-SMCSO groups, respectively, at 12-weeks, suggesting possible tissue saturation. Interestingly, two SMCSO-fed groups consume significantly more feed, without significant weight gain. Due to limitations in measuring consumed feed, caution should be taken interpreting these results. Conclusion: SMCSO (43–256 mg kg−1 BW day−1) does not ameliorate metabolic syndrome features in high-fat fed mice. Substantial knowledge gaps remain. Further studies should administer SMCSO separately (i.e., gavage), with metabolic studies exploring tissue levels to better understand its physiological action.

RAS ID

71195

Document Type

Journal Article

Date of Publication

5-1-2024

Volume

68

Issue

10

Funding Information

National Heart Foundation of Australia

PubMed ID

38704751

School

Nutrition and Health Innovation Research Institute / School of Medical and Health Sciences

Grant Number

102817

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 License.

Publisher

Wiley

Comments

Hill, C. R., Shafaei, A., Matthews, V. B., Ward, N. C., Croft, K. D., Lewis, J. R., ... & Blekkenhorst, L. C. (2024). S‐Methyl cysteine sulfoxide does not ameliorate weight gain or hyperlipidemia in mice fed a high‐fat diet. Molecular Nutrition & Food Research, 2400034. https://doi.org/10.1002/mnfr.202400034

Recommended Citation

Hill, C., Shafaei, A., Matthews, V., Ward, N., Croft, K., Lewis, J., Hodgson, J. M., Balmer, L., & Blekkenhorst, L. C. (2024). S-methyl cysteine sulfoxide does not ameliorate weight gain or hyperlipidemia in mice fed a high-fat diet. DOI: https://doi.org/10.1002/mnfr.202400034