Comparison of sodium deoxycholate and sodium dodecyl sulphate in milk fat globular membrane protein recovery

Authors/Creators

Manujaya W. Jayamanna Mohottige, Edith Cowan UniversityFollow
Angela Juhasz, Edith Cowan UniversityFollow
Mitchell G. Nye-Wood, Edith Cowan UniversityFollow
Katherine A. Farquharson
Utpal Bose, Edith Cowan UniversityFollow
Michelle L. Colgrave, Edith Cowan UniversityFollow

Abstract

This study aims to investigate detergent sodium deoxycholate (SDC) and sodium dodecyl sulphate (SDS) treatments, with a focus on each detergent's ability to enhance the extraction of immune-related proteins from camel milk fat globular membrane. Milk was separated into cream and skimmed milk. The cream was then treated using 0.4 % SDS, 2 % SDC, 6 % SDC or 10 % SDC. Extracted proteins were trypsin digested and analysed using liquid chromatography-tandem mass spectrometry. Higher SDC concentrations (6 % and 10 %) resulted in marginal improvements in protein identification (44 unique protein groups). Protein abundance distributions showed minimal differences across treatments. However, weighted gene correlation network analysis highlighted an overrepresentation of complement and coagulation cascade proteins in modules positively correlated with the aforementioned treatment order. Gene set enrichment analysis further revealed that 10 % SDC significantly enriched complement and coagulation cascade proteins and showed comparable enrichment of other immune pathways relative to the alternative detergent treatments applied.

RAS ID

81873

Document Type

Journal Article

Date of Publication

8-1-2025

Volume

144

Funding Information

Australia Research Council

School

School of Science

Grant Number

ARC Number : CE200100012

Creative Commons License

This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Publisher

Elsevier

Identifier

Manujaya W. Jayamanna Mohottige: https://orcid.org/0009-0009-8818-4054

Angela Juhasz: https://orcid.org/0000-0002-4317-2027

Mitchell G. Nye-Wood: https://orcid.org/0000-0001-9560-9916

Michelle L. Colgrave: https://orcid.org/0000-0001-8463-805X

Comments

Mohottige, M. W. J., Juhász, A., Nye-Wood, M. G., Farquharson, K. A., Bose, U., & Colgrave, M. L. (2025). Comparison of sodium deoxycholate and sodium dodecyl sulphate in milk fat globular membrane protein recovery. Journal of Food Composition and Analysis, 144, 107644. https://doi.org/10.1016/j.jfca.2025.107644

Recommended Citation

Jayamanna Mohottige, M. W., Juhasz, A., Nye-Wood, M. G., Farquharson, K. A., Bose, U., & Colgrave, M. L. (2025). Comparison of sodium deoxycholate and sodium dodecyl sulphate in milk fat globular membrane protein recovery. DOI: https://doi.org/10.1016/j.jfca.2025.107644