Influence of RFID tags on Melipona quadrifasciata behavior: Effects on locomotion and food choice
Abstract
The use of technology as a tool for evaluating insect behavior provides significant advantages to researchers, as it increases the amount of collected data, reduces observer bias, and minimizes the time and effort required for direct field observations. However, when incorporating technology into research methodologies, it is crucial to ensure that the tool does not alter the natural behavior of the studied individuals. In recent years, the use of radiofrequency electronic identification (RFID) to monitor bees has increased significantly. Yet, little is known about its potential effects on bee behavior, mainly in stingless bees. To assess the influence of electronic tags, we evaluated locomotion parameters and foraging choices by comparing feeders with and without the neonicotinoid insecticide imidacloprid in the stingless bee Melipona quadrifasciata under laboratory conditions using video tracking. Whereas the presence of electronic tags did not affect the food choice as measured by the frequency of bee visits to contaminated versus uncontaminated food sources, it did influence locomotion. Tagged bees exhibited reduced average speed, lower rotation frequency, shorter distances traveled, and altered arena exploration patterns. These behavioral modifications observed in laboratory conditions highlight important considerations for applying RFID technology in field studies monitoring bee behavior.
Document Type
Journal Article
Date of Publication
1-1-2026
Volume
234
PubMed ID
41274431
Publication Title
Behavioural Processes
Publisher
Elsevier
School
School of Engineering
Funders
Conselho Nacional de Desenvolvimento Científico e Tecnológico (PIBIC 001/2021, 400540/2018) / Fundação de Amparo à Pesquisa do Estado de São Paulo (2017/21097–3)
Copyright
subscription content
Comments
Da Silva Castor, R. E., Tadei, R., De Souza, P., Da Silva, E. C. M., & Toppa, R. H. (2025). Influence of RFID tags on Melipona quadrifasciata behavior: Effects on locomotion and food choice. Behavioural Processes, 234, 105310. https://doi.org/10.1016/j.beproc.2025.105310